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篇目详细内容 |
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【篇名】 |
Cloning and functional analysis of the genes involved in signal transduction in tomato Cf-4-Avr4 pathosystem |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-007-0009-8 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2007 |
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【卷期】 |
1
卷1期 |
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【页码】 |
52-57
页,共
6
页 |
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【作者】 |
LIU Qing;
FENG Dongxin;
WANG Xiaowu;
DU Yongchen;
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【关键词】 |
tomato; Cladosporium fulvum; hypersensitive response; yeast two-hybrid system; virus-induced gene silencing |
【摘要】 |
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Hypersensitive response (HR) is one of the most efficient and common resistance mechanisms in plants. Cloning signaling genes are very important to elucidate the resistance mechanisms. A gene in tomato homologous to several resistance proteins in plant was involved in HR and named as RGL (Resistance Gene Like). RGL protein was used as a bait to screen interacting protein (s) from tomato cDNA library through the yeast two-hybrid system. Two interacting proteins were found, which were called as RGLIP-1 and RGLIP-2 (RGL Interacting Protein), respectively. RGLIP-1 is a protein of 291 amino acids with significant homology with thylakoid lumen protein. RGLIP-2 is a protein of 248 amino acids with significant homology with transducin protein. Virus-Induced Gene Silencing (VIGS) of the two genes results in a partial and complete suppression of Avr4-induced HR, which indicates that both genes are involved in hypersensitive response. |
