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篇目详细内容 |
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【篇名】 |
Cellular immune responses of BALB/c mice induced by intramuscular injection of PRRSV-ORF5 DNA vaccine with different doses |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-007-0020-0 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2007 |
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【卷期】 |
1
卷1期 |
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【页码】 |
105-110
页,共
6
页 |
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【作者】 |
CHENG Anchun;
WANG Mingshu;
CHEN Xiwen;
XINI Nigen;
DOU Wenbo;
LI Xuemei;
LIU Wumei;
WANG Gang;
ZHANG Pingying;
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【关键词】 |
PRRSV; ORF5 DNA vaccine; cellular immune response; mice |
【摘要】 |
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BALB/c mice were immunized with 50 μg, 100 μg, 200 μg of pcDNA-PRRSV-ORF5 DNA vaccine respectively by intramuscular injection, with PBS and pcDNA3.1 (+) as controls. Fluorescence activated cell Sorter (FACS) was used to detect the number of CD4+ and CD8+ T-lymphocytes. T-lymphocyte proliferation test was used to detect proliferation of the T-lymphocyte cells in peripheral blood lymphocytes of mice vaccinated with pcDNA-PRRSV-ORF5 DNA vaccine. The results showed that the difference in ConA response to T-lymphocytes in blood was highly significant between all experimental groups and the control group (P<0.01). The number of CD4+ T-lymphocytes in experimental groups was significantly higher than that of the control group 7 d after vaccination. The number of CD8+ T-lymphocytes in the experimental groups was higher than that of the control group 28 d after vaccination. Mice immunized with a higher dose (200 μg) of DNA vaccine demonstrated higher cellular immune response than those immunized with a lower dose (100 μg, 50 μg) of DNA vaccine. The results demonstrated that pcDNA-PRRSV-ORF5 DNA vaccine could induce a good cellular immune response which may be dose-dependent. |
