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篇目详细内容 |
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【篇名】 |
Cytogenetic comparison of restorers TP-4 and Dminghui63 with maintainer D46B of autotetraploid rice |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-008-0003-9 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
2
卷1期 |
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【页码】 |
16-22
页,共
7
页 |
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【作者】 |
LONG Wenbo;
LUAN Li;
WANG Xing;
LIU Yuhua;
TU Shengbin;
KONG Fanlun;
HE Tao;
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【关键词】 |
autotetraploid rice; restorer; maintainer; meiosis; U-test; correlation analysis |
【摘要】 |
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Cytogenetical comparison was made between high seed setting restorers TP-4 and Dminghui63 with eminent maintainer line D46B of autotetraploid rice. The meiosis observation demonstrated that the genomes of our autotetraploid materials were all 2n = 48, which was the same as those in mitosis observation. Low percentages of univalent and trivalent in diakinesis-metaphase I (MI) of restorers TP-4 and Dminghui63 as well as maintainer line D46B of autotetraploid rice were observed. And the percentages of chromosome pairing were all over 99%, showing eminent cytological character. The frequency of TP-4 and Dminghui63 in diakinesis-metaphase I was 2.00/PMC (pollen mother cell) and 2.26/PMC, respectively. However, the frequency of D46B was 6.00/PMC, significantly higher than those of TP-4 and Dminghui63. It indicated that the maintainer D46B had better chromosome pairing capability in diakinesis-metaphase. While, the frequency of lagging chromosomes of the maintainer D46B in anaphase I (AI) was 10.62%, significantly lower than that of TP-4 (19.44%) or Dminghui63 (23.14%), and close to the level of diploid control (7.30%). In telophase I (TI), maintainer D46B exhibited lower frequency of microkernel, and in telophase II (TII) the frequency of normal quartered microspore of maintainer D46B was not only higher than that of TP-4 or Dminghui63 but also than that of diploid control. The percentage of the cell observed chromosome lagging in AI and the percentage of abnormal cell in TI showed a greatly significant positive correlation. That may demonstrate that chromosome separation in AI and microkernel formation in TI are controlled by the same dominant single gene or the major gene of a QTL. |
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