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篇目详细内容

【篇名】 Comparative QTL mapping of resistance to sugarcane mosaic virus in maize based on bioinformatics
【刊名】 Frontiers of Agriculture in China
【刊名缩写】 Front. Agric. China
【ISSN】 1673-7334
【EISSN】 1673-744X
【DOI】 10.1007/s11703-008-0081-8
【出版社】 Higher Education Press and Springer-Verlag
【出版年】 2008
【卷期】 2 卷4期
【页码】 365-371 页,共 7 页
【作者】 Lü Xiangling; LI Xinhai; XIE Chuanxiao; HAO Zhuanfang; JI Hailian; SHI Liyu; ZHANG Shihuang;
【关键词】 maize; sugarcane mosaic virus; quantitative trait loci; comparative gene mapping

【摘要】
The development of genomics and bioinformatics offers new tools for comparative gene mapping. In this paper, an integrated QTL map for sugarcane mosaic virus (SCMV) resistance in maize was constructed by compiling a total of 81 QTL loci available, using the Genetic Map IBM2 2005 Neighbors as reference. These 81 QTL loci were scattered on 7 chromosomes of maize, and most of them were clustered on chromosomes 3 and 6. By using the method of meta-analysis, we identified one “consensus QTL” on chromosome 3 covering a genetic distance of 6.44 cM, and two on chromosome 6 covering genetic distances of 16 cM and 27.48 cM, respectively. Four positional candidate resistant genes were identified within the “consensus QTL” on chromosome 3 via the strategy of comparative genomics. These results suggest that application of a combination of meta-analysis within a species with sequence homology comparison in a related model plant is an efficient approach to identify the major QTL and its candidate gene(s) for the target traits. The results of this study provide useful information for identifying and cloning the major gene(s) conferring resistance to SCMV in maize.
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