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篇目详细内容 |
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【篇名】 |
Cloning and sequence analysis of a mutation-type cinnamate 4-hydroxylase gene from Brassica oleracea L. var. acephala DC. |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-008-0047-x |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
2
卷4期 |
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【页码】 |
456-462
页,共
7
页 |
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【作者】 |
CHEN Anhe;
LI Jiana;
CHAI Yourong;
WANG Rui;
LU Jun;
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【关键词】 |
Brassica oleracea L. var. acephala DC.; cloning, cinnamate 4-hydroxylase (C4H); mutation; sequence analysis |
【摘要】 |
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A 2431-bp full-length cinnamate 4-hydroxylase gene, BoC4H, was cloned from Brassica oleracea L. var. acephala DC.. It contains 2 introns. Its mRNA is 1715 bp, encoding a deduced 481-amino-acid polypeptide with wide homologies to C4Hs from other plants. It possesses cytochrome P450 conserved domains and motifs such as the haem-iron binding motif, the E-R-R triad, the T-containing binding pocket motif and the hinge motif necessary for optimal orientation of the enzyme. It also has most of the canonical C4H/CYP73A5-featured substrate-recognition sites (SRSs) and active site residues. However, owing to a single-base deletion at C2242 and subsequent frame shift within the 3＇ coding region as compared with C4H genes from Arabidopsis thaliana and other plants, BoC4H shows a 36-aa deletion/variation at its C-terminus and the SRS6 motif together with active site residues therein are absent. Thus BoC4H may be of no function or low activity. BoC4H is a membrane protein and is probably associated with the endoplasmic reticulum. Its secondary structure is dominated by alpha helices and random coils. The Swiss-Model could not predict its tertiary structure. B. oleracea contains a C4H gene family with at least 5 members. |
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