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篇目详细内容 |
【篇名】 |
Fluorescence cross-correlation spectroscopy using single wavelength laser |
【刊名】 |
Frontiers of Chemistry in China |
【刊名缩写】 |
Front. Chem. China |
【ISSN】 |
1673-3495 |
【EISSN】 |
1673-3614 |
【DOI】 |
10.1007/s11458-009-0036-5 |
【出版社】 |
Higher Education Press and Springer-Verlag |
【出版年】 |
2009 |
【卷期】 |
4
卷2期 |
【页码】 |
191-195
页,共
5
页 |
【作者】 |
Chao XIE;
Chaoqing DONG;
Jicun REN;
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【关键词】 |
fluorescence cross-correlation spectroscopy; single-molecule detection; single laser excitation; quantum dot |
【摘要】 |
In this paper, we first introduced the basic principle of fluorescence cross-correlation spectroscopy (FCCS) and then established an FCCS setup using a single wavelength laser. We systematically optimized the setup, and the detection volume reached about 0.7fL. The home-built setup was successfully applied for the study of the binding reaction of human immunoglobulin G with goat antihuman immunoglobulin G. Using quantum dots (745nm emission wavelength) and Rhodamine B (580nm emission wavelength) as labeling probes and 532nm laser beam as an excitation source, the cross-talk effect was almost completely suppressed. The molecule numbers in a highly focused volume, the concentration, and the diffusion time and hydrodynamic radii of the reaction products can be determined by FCCS system. |
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