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篇目详细内容 |
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【篇名】 |
Study of two-dimensional liquid chromatography with high temperature NPLC and room temperature RPLC |
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【刊名】 |
Frontiers of Chemistry in China |
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【刊名缩写】 |
Front. Chem. China |
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【ISSN】 |
1673-3495 |
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【EISSN】 |
1673-3614 |
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【DOI】 |
10.1007/s11458-009-0033-8 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2009 |
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【卷期】 |
4
卷2期 |
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【页码】 |
196-201
页,共
6
页 |
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【作者】 |
Hongzhe TIAN;
Jing XU;
Kun DING;
Yafeng GUAN;
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【关键词】 |
two-dimensional liquid chromatography; HTNPLC /RPLC; sample loops- valve interface; traditional Chinese medicine; Glycyrrhiza uralensis |
【摘要】 |
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To overcome the peak band broadening and to increase the peak capacity and separation efficiency of a two-dimensional liquid chromatographic system, a high-temperature normal phase liquid chromatography (HTNPLC) was used as the first dimension (1st-D), and a RPLC was used as the second dimension (2nd-D). The sample was first separated on the 1st-D CN column and the primary eluent stored in the sampling-loop system alternatively (in HTNPLC×RPLC mode) or selectively (in HTNPLC/RPLC mode) and was then transferred to 2nd-D C18 column for further separation. The resolution and separation efficiency of the systems were greatly improved. The systems were evaluated by analyzing several polycyclic aromatic hydrocarbons and Glycyrrhiza uralensis extract. |
