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篇目详细内容 |
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【篇名】 |
Sample pretreatment microfluidic chip for DNA extraction from rat peripheral blood |
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【刊名】 |
Frontiers of Chemistry in China |
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【刊名缩写】 |
Front. Chem. China |
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【ISSN】 |
1673-3495 |
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【EISSN】 |
1673-3614 |
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【DOI】 |
10.1007/s11458-007-0015-7 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2007 |
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【卷期】 |
2
卷1期 |
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【页码】 |
74-78
页,共
5
页 |
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【作者】 |
CHEN Xing;
CUI Dafu;
LIU Changchun;
LI Hui;
ZHAO Weixing;
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【关键词】 |
micro electro mechanical system; oxidized porous silicon; sample pretreatment microfluific chip |
【摘要】 |
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A sample pretreatment microfluidic chip was described based on the principle of solid phase extraction and micro electro mechanical system technology. Oxidized porous silicon with the large surface area as the solid phase matrix for absorption of DNA from a biological sample can greatly improve the DNA yield. The factors that could affect the DNA yield were analyzed and the preparation technology and the experiment procedure were improved. The DNA purification process from the rat peripheral blood can be achieved and the DNA yield is 24 ng/(μL whole blood), which can reach the level of the commercial DNA purification kits. Furthermore, the DNA extracted from the whole blood can be amplified by polymerase chain reaction, which can achieve a high efficiency of the amplification. |
