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篇目详细内容 |
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【篇名】 |
Identification and characterization of supercritical fluid extracts of Rhizoma Chuanxiong by high performance liquid chromatography ion trap mass spectrometry |
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【刊名】 |
Frontiers of Chemistry in China |
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【刊名缩写】 |
Front. Chem. China |
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【ISSN】 |
1673-3495 |
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【EISSN】 |
1673-3614 |
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【DOI】 |
10.1007/s11458-006-0070-5 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2006 |
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【卷期】 |
1
卷4期 |
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【页码】 |
454-458
页,共
5
页 |
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【作者】 |
Teng Jiuwei;
Chen Hongwei;
Li Deliang;
Luo Andong;
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【关键词】 |
HPLC-MS/MS; Rhizoma Chuanxiong; ligustilide; CID; supercritical fluid extraction |
【摘要】 |
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The main constituents, senkyunolide A, Z-ligustilide, neocnidilide, 3-butylphthalide, and ligustilide dimers, in supercritical CO2 fluid extracts of Rhizoma Chuanxiong, a popular Chinese traditional medicine, have been identified and characterized by high performance liquid chromatography tandem mass spectrometry. Separations were carried out on an Agilent (ECLIPSE XDB) C18 analytical column by gradient elution with 0.25% acetic acid and methanol (containing 0.25% acetic acid). An Agilent 1100 series LC/MSD XCT system was operated under positive ESI and auto MS/MS modes for mass spectrometric analysis. Collision- induced dissociation (CID) fragmentations of these phthalides have been investigated and elucidated. Phthalides have primarily undergone two ESI CID pathways: side-chain cleavage with losses of alkenes and ring-opening with eliminations of H2O followed by losses of CO. Direct neutral loss of CO has not been observed. Sodium adduct ions have demonstrated completely different CID pathways. |
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