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篇目详细内容 |
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【篇名】 |
Dissection of gene function at clonal level using mosaic analysis with double markers |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-013-1279-6 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2013 |
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【卷期】 |
8
卷6期 |
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【页码】 |
557-568
页,共
12
页 |
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【作者】 |
Simon HIPPENMEYER;
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【关键词】 |
MADM; genetic mosaic; clonal analysis; lineage tracing; neural development; genomic imprinting |
【摘要】 |
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MADM (Mosaic Analysis with Double Markers) technology offers a genetic approach in mice to visualize and concomitantly manipulate genetically defined cells at clonal level and single cell resolution. MADM employs Cre recombinase/loxP-dependent interchromosomal mitotic recombination to reconstitute two split marker genes—green GFP and red tdTomato — and can label sparse clones of homozygous mutant cells in one color and wild-type cells in the other color in an otherwise unlabeled background. At present, major MADM applications include lineage tracing, single cell labeling, conditional knockouts in small populations of cells and induction of uniparental chromosome disomy to assess effects of genomic imprinting. MADM can be applied universally in the mouse with the sole limitation being the specificity of the promoter controlling Cre recombinase expression. Here I review recent developments and extensions of the MADM technique and give an overview of the major discoveries and progresses enabled by the implementation of the novel genetic MADM tools. |
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