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篇目详细内容 |
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【篇名】 |
The effect of ethylene inhibitors (AgNO3, AVG), an ethylene- liberating compound (CEPA) and aeration on the formation of protocorm-like bodies of hybrid Cymbidium (Orchidaceae) |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-013-1283-x |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2013 |
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【卷期】 |
8
卷6期 |
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【页码】 |
606-610
页,共
5
页 |
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【作者】 |
Jaime A.;
TEIXEIRA DA SILVA;
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【关键词】 |
AgNO3; AVG; CEPA; PLB; Teixeira Cymbidium (TC) medium |
【摘要】 |
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Protocorm-like bodies (PLBs) or thin cell layers (TCLs) derived from PLBs of hybrid Cymbidium Twilight Moon ‘Day Light’ can induce new or neo-PLBs on Teixeira Cymbidium (TC) medium, which contains 0.1？mg/L α-naphthaleneacetic acid, 0.1？mg/L kinetin, 2？g/L tryptone and 20？g/L sucrose, and is solidified with 8？g/L Bacto agar. This study aimed to assess the response of neo-PLB formation to an ethylene-liberating compound (2-chloroethylphosphonic acid (CEPA)), to two ethylene inhibitors (silver nitrate (AgNO3) and aminoethoxyvinylglycine (AVG)), and to aeration (made possible by using Milliseal？ or autoclaved filter paper). AgNO3 at 1 or 2？mg/L in TC medium significantly increased the fresh weight of PLBs while 1？mg/L of AgNO3 also showed a significant increase in the number of neo-PLB from both half-PLBs and from tTCLs. In contrast, AVG and CEPA inhibited neo-PLB formation. Neo-PLB formation from half-PLB or TCL explants in the presence of aeration resulted in significantly lower neo-PLB weight. The use of AgNO3 and aeration are alternative means to mass produce neo-PLBs for micropropagation purposes. |
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