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篇目详细内容 |
【篇名】 |
Screening and molecular characterization of Serratia marcescens VITSD2: A strain producing optimum serratiopeptidase |
【刊名】 |
Frontiers in Biology |
【刊名缩写】 |
Front. Biol.
|
【ISSN】 |
1674-7984 |
【EISSN】 |
1674-7992 |
【DOI】 |
10.1007/s11515-013-1284-9 |
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
【出版年】 |
2013 |
【卷期】 |
8
卷6期 |
【页码】 |
632-639
页,共
8
页 |
【作者】 |
C.;
Subathra DEVI;
Renuka ELIZABETH JOSEPH;
Harini SARAVANAN;
S.;
Jemimah NAINE;
V.;
Mohana SRINIVANSAN;
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【关键词】 |
Serratiopeptidase; Serratia marcescens VITSD2; antibacterial activity |
【摘要】 |
The current work was attempted to isolate and characterize the serratiopeptidase producing Serratia sp. Among the 10 bacterial isolates 7 strains were identified as Serratia sp. Out of 7 strains one showed potent proteolytic activity and selected for further studies. Based on the morphological, biochemical and molecular characterization, the potent isolate (RH03) was identified as Serratia marcescens (GenBank accession number: KC961637) and the strain was designated as Serratia marcescens VITSD2. The production of serratiopeptidase was carried out in trypticase soya broth and the enzyme was partially purified using ammonium sulfate precipitation and dialysis. The specific activity was determined by casein hydrolysis assay and was found to be 12.00, 21.33, and 25.40?units/mg for crude, precipitated and dialysed samples. The molecular weight of the protease was determined by SDS-PAGE and it was found to be 50?kDa. The antibacterial activity of the produced serratiopeptidase showed moderate activity against Pseudomonas aeruginosa MTCC No. 4676 (12 mm) and Escherichia coli MTCC No. 1588 (15 mm). |
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