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篇目详细内容 |
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【篇名】 |
Single-cell genomics: An overview |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-013-1285-8 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2013 |
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【卷期】 |
8
卷6期 |
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【页码】 |
569-576
页,共
8
页 |
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【作者】 |
Qichao WANG;
Xianmin ZHU;
Yun FENG;
Zhigang XUE;
Guoping FAN;
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【关键词】 |
single-cell genomics; next-generation sequencing; RNA-seq; single-nucleotide variation; copy-number variation; DNA methylation |
【摘要】 |
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The newly developed next-generation sequencing platforms, in combination with genome-scale amplification methods, provide a powerful tool to study genomics from a single cell. This mini-review summarizes the technologies of single cell genomics and their applications in several areas of biomedical research including stem cells, cancer biology and reproductive medicine. Particularly, it highlights recent advances in single cell exome sequencing, RNA-seq, and genome sequencing. The application of these powerful techniques will shed new light on the fundamental principles of gene transcription and genome organization at single-cell level and improve our understanding of cellular heterogeneity and diversity in multicellular organisms. |
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