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篇目详细内容 |
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【篇名】 |
Ultraviolet-B induced expression of hypoxia-inducible factor 1α, transferrin receptor through EGFR/PI3K/AKT/DEC1 pathway |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-007-0016-4 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2007 |
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【卷期】 |
1
卷1期 |
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【页码】 |
79-86
页,共
8
页 |
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【作者】 |
LI Yanhua;
BI Zhigang;
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【关键词】 |
ultraviolet-B; hypoxia-inducible factor 1α; receptors, transferrin; receptors, epidermal growth factor; phosphatidylinositol-3-kinase; DEC1 |
【摘要】 |
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The aim of this research was to explore the effects and signaling pathway of ultraviolet-B (UVB) irradiation on the expression of hypoxia-inducible factor 1α (HIF-1α) and transferrin receptor (TfR). HIF-1α protein was measured by Western blot method. Expressions of epidermal growth factor receptor (EGFR), phosphor-EGF-R and TfR after UVB irradiation were determined with flow cytometry. After UVB irradiation, mRNA levels of HIF-1α and TfR were detected by real time-PCR. Results showed that compared with control groups, UVB was able to induce HIF1α and TfR protein expression in a dose- and time-dependent manner in HaCat cells (P<0.05). TfR mRNA was expressed in a dose-dependent manner and reached a peak at the 8th hour in HaCat cells (P<0.05) whereas HIF-1α mRNA expression was not affected by UVB treatment (P>0.05). The EGFR/PI3K/AKT signaling pathway was required for the induction of HIF-1α and TfR expression induced by UVB. UVB induced activation of EGFR in HaCat cells and EGFR regulated expression of TfR and HIF-1α. EGFR (?/?) MEF did not increase the HIF1 expression following UVB irradiation (P>0.05). In contrast, EGFR (+/+) MEF strongly enhanced HIF1a expression after UVB irradiation (P<0.05). PD153035, a selective inhibitor of EGFR tyrosine kinase, inhibited the TfR protein expression in UVB-treated cells in a dose-dependent manner (P<0.05). PI3K inhibitors, LY294002 and wortmannin, inhibited HIF-1α and TfR expressions induced by UVB (P<0.05). The DEC1 (?/?) Ha-Cat cells did not increase their TfR and HIF-1α expressions following UVB irradiation (P>0.05). In contrast, DEC1 (+/+) HaCat cells strongly enhanced TfR and HIF-1α protein expression after UVB irradiation (P<0.05). We conclude that UVB induces TfR and HIF-1α expressions via EGFR/PI3K/AKT/DEC1 signaling pathway. |
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