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篇目详细内容 |
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【篇名】 |
Involvement of p38 mitogen-activated protein kinase in the regulation of platelet-derived growth factor -induced cell migration |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-007-0047-x |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2007 |
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【卷期】 |
1
卷3期 |
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【页码】 |
248-252
页,共
5
页 |
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【作者】 |
GONG Xiaowei;
WEI Jie;
LI Yusheng;
CHENG Weiwei;
DENG Peng;
JIANG Yong;
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【关键词】 |
p38 mitogen-activated protein kinase; plateletderived growth factor; cell movement; cytoskeleton; signal transduction |
【摘要】 |
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The aim of this study was to investigate the role of p38 mitogen-activated protein kinase (MAPK) in cell migration induced by platelet-derived growth factor (PDGF). Western blot was performed to detect the phosphorylation of p38 in NIH3T3 cells treated with PDGF. A Transwell cell migration system was used to determine the effects of PDGF treatment on the migration of NIH3T3 cells and the influence of p38 deficiency on this process in a p38 gene knockout (p38?/?) mouse embryonic fibroblast cell line. On the stimulation of PDGF, the migration of NIH3T3 cells was significantly increased (P<0.001) compared to the control and p38 MAP kinase was simultaneously phosphorylated. Furthermore, the PDGF-induced cell migration was significantly blocked in p38 gene knockout (p38?/?) mouse embryonic fibroblasts (MEFs) (P<0.001) as compared with the wild type cells (p38+/+). p38 MAPK plays an important role in the regulation of cell migration induced by PDGF. |
