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篇目详细内容 |
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【篇名】 |
Kinetic analysis of interaction between lipopolysaccharide and biomolecules |
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【刊名】 |
Frontiers of Chemistry in China |
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【刊名缩写】 |
Front. Chem. China |
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【ISSN】 |
1673-3495 |
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【EISSN】 |
1673-3614 |
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【DOI】 |
10.1007/s11458-008-0005-4 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
3
卷1期 |
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【页码】 |
14-17
页,共
4
页 |
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【作者】 |
YANG Fan;
YANG Xiurong;
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【关键词】 |
Lipopolysaccharide; biomolecule; surface plasmon resonance; interaction |
【摘要】 |
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Lipopolysaccharide (LPS) is a major component of the outer membrane of all gram-negative bacteria. It interacts with some biomolecules and triggers a toxic reaction. In this paper, we studied the interaction between LPS from Salmonella Minnesota and some biomolecules using a surface plasmon resonance (SPR) biosensor. Biomolecules were immobilized on a CM5 sensor chip using the amino coupling method and LPS was injected over the immobilized surfaces. The affinity constant KA of LPS with serum albumin, hemoglobin, chitosan and lysozyme was 2.36 × 107, 2.03 × 108, 7.58 × 106, 2.82 × 104 L·mol-1, respectively. However, LPS could not interact with ferritin. |
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