|
篇目详细内容 |
【篇名】 |
Effects of pentoxifylline, platelet activating factor and prostaglandin F 2-alpha on Giant Panda (Ailuropoda melanoleuca) post-thawed sperm in vitro fertilizing capability |
【刊名】 |
Frontiers of Agriculture in China |
【刊名缩写】 |
Front. Agric. China |
【ISSN】 |
1673-7334 |
【EISSN】 |
1673-744X |
【DOI】 |
10.1007/s11703-008-0029-z |
【出版社】 |
Higher Education Press and Springer-Verlag |
【出版年】 |
2008 |
【卷期】 |
2
卷2期 |
【页码】 |
229-236
页,共
8
页 |
【作者】 |
ZHANG Ming;
XIAN Hong;
HOU Rong;
LAI Songjia;
ZHU Qing;
|
【关键词】 |
pentoxifylline; platelet activating factor; prostaglandin F 2-alpha; Giant Panda; post-thawed sperm; fertilizing capability |
【摘要】 |
Is it possible that Giant Panda (Ailuropoda melanoleuca) post-thawed sperm fertilization is improved with pentoxifylline (PF), platelet activated factor (PAF) and prostaglandin F 2-alpha (PGF2α)? In our study Giant Panda post-thawed sperm was incubated in Ham’s F-10 medium with different concentration of PF, PAF and PGF2α under 37°C. The effects of PF, PAF and PGF2α on Giant Panda post-thawed sperm fertility were evaluated through sperm motility, survival time, sperm membrane integrity, acrosome state and heterospecific egg penetration. The results were that PF, PAF and PGF2α all can affect Giant Panda post-thawed sperm in vitro fertilizing capability. In the experiment: 1 mg·mL-1 PF was most suitable for improving Giant Panda post-thawed sperm in vitro fertilizing capability. In the 1 mg·mL-1 PF group, sperm survival time was (15.33 ± 4.73) h, the heterospecific egg penetration was 51.44% after incubating for 4 hours, the heterospecific egg penetration was 7.49% after incubating for 6 hours. The results of the 1 mg·mL-1 PF group were significantly higher than those of the control group (P < 0.01). The results were also higher than those of the other treatment groups; Treated Giant Panda post-thawed sperm with 50 ng·mL-1 PAF had a better effect than 100 ng·mL-1 PAF, but the sperm fertilizing capability was damaged when incubation time exceeded 2 hours; 50 ng·mL-1 PGF2α had no significant effect on Giant Panda post-thawed sperm, but when the PGF2α treated concentration was increased, sperm in vitro fertilizing capability decreased because of the damaged motility and declined acrosomal reaction rate. The conclusions suggest that it is possible to improve post-thawed Giant Panda sperm fertility with 1 mg·mL-1 PF. |
|