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篇目详细内容 |
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【篇名】 |
Genetic variation of the genus Kengyilia by ISSR markers |
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【刊名】 |
Frontiers in Biology(Formerly known as Frontiers of Biology in China) |
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【刊名缩写】 |
Front. Biol. |
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-008-0022-1 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
3
卷2期 |
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【页码】 |
170-175
页,共
6
页 |
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【作者】 |
ZHANG Li;
ZHOU Yonghong;
DING Chunbang;
YANG Ruiwu;
LIU Shigui;
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【关键词】 |
Kengyilia; ISSR markers; genetic variation |
【摘要】 |
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We investigated the genetic variation within 32 accessions distributed to 14 species and one variety by using ISSR (inter-simple sequence repeat) markers. The results showed that genetic variation was relatively higher among the accessions. A total of 593 bands were amplified by 12 ISSR primers, of which 535 bands (90.2%) were polymorphic. Eleven to 80 polymorphic bands were amplified from each prime, with an average of 44.6 bands. The interspecies GS (genetic similarity) value ranged from 0.430 to 0.866, and the average was 0.620. Cluster analysis showed that all accessions could be classified into 4 groups by ISSR markers. The different accessions in a species were clustered together, but they had genetic variation in molecular levels. There was obvious interspecies genetic variation. Species with similar morphological characteristics and from the same areas or neighboring geographical regions were clustered together and had close relationships. ISSR markers are useful in analyzing interspecies variation in Kengyilia. |
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