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篇目详细内容 |
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【篇名】 |
Multicolor FISH analysis of rDNA and telomere on spinach |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-008-0059-6 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
2
卷3期 |
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【页码】 |
314-316
页,共
3
页 |
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【作者】 |
LAN Tianying;
LIU Bo;
DONG Fengping;
CHEN Ruiyang;
LI Xiulan;
CHEN Chengbin;
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【关键词】 |
spinach; multicolor FISH; rDNA, telomere |
【摘要】 |
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In this study, multicolor fluorescence in situ hybridization (FISH) analysis on metaphase chromosomes of spinach with biotin-labeled 25S rDNA, DIG-labeled telomere sequences and biotin-labeled and DIG-labeled 5S rDNA was performed. There were six 25S rDNA loci located on the satellites of the third, the fifth and the sixth chromosomes, and four 5S rDNA loci located on the long arms of the third and the fifth chromosomes. The telomere loci were located on the end of the sixth chromosome and also on both the end and centromeric regions of other chromosomes. This study is an important complement to both traditional karyotype analysis and FISH karyotype analysis in spinach. |
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