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篇目详细内容 |
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【篇名】 |
Changes of phenotype and function of human CD4+ CD25- T cells induced by transfection of Foxp3 |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-008-0070-6 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2008 |
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【卷期】 |
2
卷4期 |
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【页码】 |
366-369
页,共
4
页 |
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【作者】 |
WU Kui;
BI Yutian;
WANG Yaoli;
WANG Changzheng;
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【关键词】 |
Forkhead transcription factors; FOXP3 protein, human; T-lymphocytes, regulatory; Retroviridae |
【摘要】 |
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The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and function of naive CD4+ T cells. The pMSCV-Foxp3 retroviral vector encoding Foxp3 gene was transduced into the PT67 packaging cell line. Virus-containing supernatant was applied to differentiate CD4+CD25- T cells. The resulting cells were sorted with flow cytometry. The expressions of CD25, CD127, CTLA-4 and the proliferation of transfected T cells were examined. The effect of transfected CD4+ T cells on the proliferation and cytokine production of CD4+CD25- T cells was examined. Foxp3-gene transfected CD4+ T cells could express Foxp3 and transfection of Foxp3 gene up-regulated the expressions of CD25 and CTLA-4, but down-regulated CD127 expression. After transfection, the proliferation of CD4+ T cells was eliminated. Transfected T cells inhibited the proliferation of CD4+CD25- T cells. CD4+CD25- T cells acquired a regulatory phenotype and function after it was transduced with the Foxp3 gene. This suggested a key role of Foxp3 in the generation of CD4+CD25+ regulatory T cells. |
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