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篇目详细内容 |
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【篇名】 |
Detection of Eperythrozoon wenyoni by PCR assay |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-009-0014-1 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2009 |
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【卷期】 |
3
卷1期 |
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【页码】 |
100-103
页,共
4
页 |
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【作者】 |
Jian WANG;
Yutao ZHU;
Jianhua QIN;
Jian WANG;
Fumei ZHANG;
Yuelan ZHAO;
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【关键词】 |
cattle; Eperythrozoon wenyoni; PCR |
【摘要】 |
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The objective of this research was to develop a detection method for Eperythrozoon wenyoni infection using polymerase chain reaction (PCR) assay technique. A pair of primers was designed and synthesized according to the conservative sequence 16S rRNA. The PCR assay was performed with the primers. A 985-bp fragment was amplified by using PCR. The amplified fragments with the expected size were identified by EcoR I restriction digestion. The crossing-reaction, specific-reaction and duplicate-reaction indicated that the PCR method is a specific, sensitive, fast and effective method for diagnosing E. wenyoni infection at group level. |
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