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篇目详细内容 |
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【篇名】 |
Establishment and drug sensitivity evaluation of murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L6) |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-009-0022-9 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2009 |
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【卷期】 |
3
卷2期 |
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【页码】 |
119-129
页,共
11
页 |
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【作者】 |
Hongying ZHANG PhD;
Jianwu TANG MM;
Wenting ZHU BM;
Chunxiu HU MS;
Guowang XU PhD;
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【关键词】 |
murine ascites hepatocarcinoma cell line; metastasis; curcumin; drug sensitivity |
【摘要】 |
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In order to provide a sensitive cell line model for investigating the mechanisms underlying the lymphatic metastasis of tumors and the effect of medicine against cells, a new murine ascites hepatocarcinoma cell line with high lymphatic metastatic potential (Hca-P/L6) was established and the effect of curcumin on biological behavior of Hca-P/L6 was observed. Murine ascites hepatocarcinoma cell strain with low lymphatic metastatic potential (Hca-P) was subcutaneously inoculated into the medioventral line of a mouse 615 and the first generation of metastatic tumor cells of inguinal lymph node (Hca-P/L1) was obtained. Then, Hca-P/L1 was screened by the route of mouse foot pad subcutaneously → lymph node → scale-up culture in vitro → mouse foot pad subcutaneously for five times consecutively. The sensitivity of two murine ascites hepatocarcinoma cell lines (Hca-P and Hca-P/L6) and two anchorage-dependent human hepatocarcinoma cell lines (SMC7721 and HepG2) to curcumin were studied by use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay after these cells had been pretreated by curcumin at the concentration of 15─240μmol/L for 48 h. After pretreatment by curcumin at the maximum non-cytotoxic dose of 15μmol/L in vitro, the effect of curcumin against cell proliferation of Hca-P and Hca-P/L6 was observed by inverted microscope, cell growth curve and cell population doubling time; the effects of curcumin on cell cycles of Hca-P/L6 and Hca-P were studied by flow cytometry (FCM). The results showed Hca-P/L6 spreading to the lymph nodes at multiple sites in mice was screened from Hca-P. The lymph node metastatic rate was 100%. Curcumin had significant growth inhibiting effect on both murine ascites and human hepatocarcinoma cell lines in a dose-dependent manner (P<0. 05). At concentrations of 30─120μmol/L, curcumin had more inhibition on murine ascites hepatocarcinoma cell lines than on human anchorage-dependent hepatocarcinoma cell lines. At concentrations of 60─240μmol/L, curcumin had more inhibition on Hca-P/L6 with (the 50% inhibitory concentration) IC50 of 51.48μmol/L than on Hca-P with IC50 of 90.87μmol/L. After pretreatment by curcumin at the maximum non-cytotoxic dose of 15mol/L for 7 days, the proliferations of Hca-P/L6 and Hca-P were inhibited (P<0.05) in a time-dependent manner (P<0.01) and the population doubling time of Hca-P/L6 and Hca-P was prolonged (P<0.01), and curcumin had more inhibition on Hca-P/L6 than on Hca-P (P<0.05). After pretreatment by 15μmol/L curcumin for 48h, the morphous of Hca-P/L6 was influenced more seriously than that of Hca-P and the cell cycle was redistributed with Hca-P/L6 being blocked in the S phase and Hca-P in the S and G2/M phases. Hca-P/L6 was validated to be more sensitive to curcumin than Hca-P. Hca-P/L6 is a novel sensitive cell line model for investigating the mechanisms underlying tumor lymphatic metastasis and the effect of the medicine against cells. |
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