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篇目详细内容 |
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【篇名】 |
Design of tandem genes cluster for isoflavone engineering |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-009-0057-y |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2009 |
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【卷期】 |
3
卷3期 |
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【页码】 |
292-296
页,共
5
页 |
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【作者】 |
Xunli XIA PhD;
Guangxiao YANG PhD;
Guangyuan HE PhD;
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【关键词】 |
isoflavones engineering; secondary metabolism; tandem genes cluster |
【摘要】 |
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In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected. |
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