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篇目详细内容 |
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【篇名】 |
Establishment and application of bioassay method for screening fungicides against cucumber powdery mildew |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-009-0066-2 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2009 |
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【卷期】 |
3
卷4期 |
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【页码】 |
425-430
页,共
6
页 |
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【作者】 |
Hongmin REN;
Yanan WANG;
Jianjian WEI;
Keqiang CAO;
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【关键词】 |
Sphaerotheca fuliginea Poll.; cucumber powdery mildew; bioassay system; chrysophanol |
【摘要】 |
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Sphaerotheca fuliginea Poll., a pathogen causing powdery mildew in cucumber, was used as a target for establishing a bioassay method for screening fungicides. Different cucumber varieties, seedling ages, spore culturing times, and inoculation concentrations were tested. The results showed that a highly susceptible variety “Xinchangchunmici” was a suitable cultivar for bioassay test, with the appropriate seedling age of 5─10 d, the spore culturing-time of 10─15 d and the spore concentration of 30─40 spores per field of vision (10 × 10 times under microscope) in the suspension. Cotyledon-spraying method, and leaf-disc method were compared for bioassay and Trypan Blue staining method was used for detecting thedevelopment of S. fuliginea on the leaf surface. Based on the bioassay system established above, the protective effect of chrysophanol on the development of S. fuliginea was evaluated using cotyledon-spraying method. The results showed that chrysophanol reduced the disease index effectively and the protective EC50 value was 33.36 μg·mL−1. |
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