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篇目详细内容 |
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【篇名】 |
Immobilization of penicillin G acylase onto amino-modified silica hydrogel |
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【刊名】 |
Frontiers of Chemical Engineering in China |
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【刊名缩写】 |
Front. Chem. Eng. China |
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【ISSN】 |
1673-7369 |
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【EISSN】 |
1673-7474 |
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【DOI】 |
10.1007/s11705-009-0298-y |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2010 |
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【卷期】 |
4
卷1期 |
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【页码】 |
87-90
页,共
4
页 |
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【作者】 |
Weibing Dong;
Huining HE;
Junbo Gong;
Victor C. YANG;
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【关键词】 |
mesoporous materials; covalent immobilization; penicillin G acylase; amino-modified silica gel |
【摘要】 |
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Amino-modified silica hydrogel (N-MSHG) was prepared by a simple sol-gel processing via the co-condensation of commercial silica sol with 3-aminopropyltrie-oxysilane. Penicillin G acylase (PGA), a model enzyme, was covalently immobilized onto the N-MSHG and then was used for the enzymatic synthesis of amoxicillin. The samples were characterized by Nitrogen sorption analysis, FT-IR and thermal gravimetric analysis (TGA). The results showed that the amino-modified gel was a mesoporous material with an average pore size of 12.64±0.17nm. The immobilization process was efficient and the immobilized enzyme showed high catalytic efficiency. The yield of the synthesis of amoxicillin in aqueous media was 38% for 2.5h. This sol-gel preparation is simple and shows prominent potential value in industrial processing. |
