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篇目详细内容 |
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【篇名】 |
Inhibition of NF-kappa B can enhance Fas-mediated apoptosis in leukemia cell line HL-60 |
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【刊名】 |
Frontiers of Medicine in China |
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【刊名缩写】 |
Front. Med. China |
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【ISSN】 |
1673-7342 |
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【EISSN】 |
1673-7458 |
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【DOI】 |
10.1007/s11684-010-0026-5 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2010 |
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【卷期】 |
4
卷3期 |
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【页码】 |
323-328
页,共
6
页 |
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【作者】 |
Li WANG MM;
Shi ZHAO MD;
Hong-Xiang WANG MD;
Ping ZOU MD;
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【关键词】 |
nuclear factor-kappa B; Fas/FasL system; HL-60; Bay 11-7082 |
【摘要】 |
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This study explored the effects of nuclear factor-kappa？ ？B？？ (NF-κB) ？？inhibitor？？ Bay？？ 11-7082？？ on Fas/FasL system and Fas-mediated apoptosis in cell line HL-60 cells. The mRNA and protein levels of Fas, FasL, and X-linked inhibitor of apoptosis protein (XIAP) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM); the level of sFasL was evaluated by enzyme-linked immunosorbent assay (ELISA); and apoptosis was determined by FCM. After treatment with Bay 11-7082, the mRNA and protein levels of FasL and XIAP in HL-60 cells were significantly lower than in the controls (P<0.05), but the mRNA and protein levels of Fas and sFasL did not change significantly (P>0.05). Apoptotic rate of HL-60 cells treated with Bay 11-7082 was significantly higher than in the controls (P<0.05). Therefore, we conclude that Bay 11-7082 can enhance Fas-mediated apoptosis in HL-60 cells by downregulating FasL and XIAP levels. |
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