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篇目详细内容 |
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【篇名】 |
Construction of a plant expression vector of chalcone synthase gene of Ginkgo biloba L. and its genetic transformation into tobacco |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-010-1033-7 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2010 |
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【卷期】 |
4
卷4期 |
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【页码】 |
456-462
页,共
7
页 |
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【作者】 |
Linling LI;
Hua CHENG;
Jianying PENG;
Shuiyuan CHENG;
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【关键词】 |
chalcone synthase gene; Ginkgo biloba L.; plant expression vector; flavones |
【摘要】 |
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The chalcone synthase gene (chs) from Ginkgo biloba L. was cloned by PCR procedure. For constructing a plant expression vector of Gbchs, the gene was digested with XbaI and BamHI and inserted into the pBI121 vector. Gbchs was transferred into tobacco mediated by Agrobacterium tumefaciens LBA4404. PCR and Southern blot were performed, and the results showed that chs had been transformed into the genomic DNA of tobacco. The total flavones in the transformed tobacco leaves was extracted by the methanol extraction method and tested by UV spectrophotometry. The results showed that, compared to the control tobacco, the content in the transgenic tobaccos, n=6, was generally higher, and the difference reached a significant level (P<0.05) and highly significant level (P<0.01), except one sample. Moreover, the highest sample was nearly 7.7 times greater than that of the controls. All these results show that utilizing genetic manipulations to improve plants in order to regulate flavone content by gene engineering may be an effective and hopeful method. |
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