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篇目详细内容 |
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【篇名】 |
Protein extraction from rice (Oryza sativa L.) root for two-dimensional electrophresis |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-010-1031-9 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2010 |
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【卷期】 |
4
卷4期 |
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【页码】 |
416-421
页,共
6
页 |
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【作者】 |
Xiaoliang XIANG;
Shuju NING;
Xia JIANG;
Xiaogui GONG;
Renlei ZHU;
Lanfang ZHU;
Daozhi WEI;
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【关键词】 |
protein extraction; two-dimensional electrophresis (2-DE); rice (Oryza sativa L.) roots |
【摘要】 |
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An efficient protein extraction method for two-dimensional gel electrophoresis (2-DE) from plant samples is usually challenging due to the low protein content and high level of interfering compounds. Proteomic analyses of rice (Oryza sativa L.) roots are limited by the lack of an efficient protein extraction method. To establish an effective protocol of protein extraction suitable for 2-DE analysis in rice roots, we evaluated three protein extraction methods (trichloroacetic acid [TCA]/acetone, Mg/NP-40/TCA, and tris-base/acetone). Our results showed that the Mg/NP-40/TCA extraction method had the highest protein yield and is the best resolution of protein separation among the three methods. The TCA/acetone method exhibited clear ？protein？ profiles？ and ？detected？ more ？protein ？spots with the highest intensity in the region of high Mr (above 45？kDa) than the other methods. However, this method was unable to detect proteins with low-Mr (less than 24.0？kDa). The Tris-base/acetone method showed the poorest resolution of protein separation. Our results suggest that the Mg/NP-40/TCA method was the most effective among the three methods and may provide enhanced proteomic information for rice and other crop roots. |
