篇目详细内容 |
【篇名】 |
Studies on isolated microspore embryoid induction of C. annuum L. |
【刊名】 |
Frontiers of Agriculture in China |
【刊名缩写】 |
Front. Agric. China |
【ISSN】 |
1673-7334 |
【EISSN】 |
1673-744X |
【DOI】 |
10.1007/s11703-010-1028-4 |
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
【出版年】 |
2010 |
【卷期】 |
4
卷4期 |
【页码】 |
438-442
页,共
5
页 |
【作者】 |
Ting YIN;
Song TIAN;
Shuangxia LUO;
Xueping CHEN;
Yanhua WANG;
Shuxing SHEN;
|
【关键词】 |
Capsicum annuum L.; isolated-microspore culture; embryoids; embryoid induction rate; double-layer medium system |
【摘要】 |
A system to improve isolated microspore embryoid induction rate of pepper (Capsicum annuum L.) was studied in this paper. The results showed that low-temperature pretreatment, growth regulators combinations, activated carbon concentrations, and preculture temperatures were critical factors affecting embryoid formation of isolated pepper microspores in vitro. One day after pretreatment of the buds at 4癈, the anthers that differentiated and developed into embryos were cultured in double-layer medium system for one week at 7癈 and then went on culturing at 28癈 in darkness. All the seven genotypes of the tested pepper responded to this protocol. The embryoid induction rate of the best genotype increased from 81.11% to 147.22%. This protocol can be used as a potential tool for producing doubled haploid plants for pepper breeding. |