|
|
|
篇目详细内容 |
|
【篇名】 |
Microtubule-associated deacetylase HDAC6 promotes angiogenesis by regulating cell migration in an EB1-dependent manner |
|
【刊名】 |
Protein & Cell |
|
【刊名缩写】 |
Protein Cell
|
|
【ISSN】 |
1674-800X |
|
【EISSN】 |
1674-8018 |
|
【DOI】 |
10.1007/s13238-011-1015-4 |
|
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
|
【出版年】 |
2011 |
|
【卷期】 |
2
卷02期 |
|
【页码】 |
150-160
页,共
11
页 |
|
【作者】 |
Dengwen Li;
Songbo Xie;
Yuan Ren;
Lihong Huo;
Jinmin Gao;
Dandan Cui;
Min Liu;
Jun Zhou;
|
|
【关键词】 |
angiogenesis; histone deacetylase 6 (HDAC6); cell migration; cell polarization; microtubule end binding protein 1 (EB1) |
【摘要】 |
|
Angiogenesis, a process by which the preexisting blood vasculature gives rise to new capillary vessels, is associated with a variety of physiologic and pathologic conditions. However, the molecular mechanism underlying this important process remains poorly understood. Here we show that histone deacetylase 6 (HDAC6), a microtubule-associated enzyme critical for cell motility, contributes to angiogenesis by regulating the polarization and migration of vascular endothelial cells. Inhibition of HDAC6 activity impairs the formation of new blood vessels in chick embryos and in angioreactors implanted in mice. The requirement for HDAC6 in angiogenesis is corroborated in vitro by analysis of endothelial tube formation and capillary sprouting. Our data further show that HDAC6 stimulates membrane ruffling at the leading edge to promote cell polarization. In addition, microtubule end binding protein 1 (EB1) is important for HDAC6 to exert its activity towards the migration of endothelial cells and generation of capillary-like structures. These results thus identify HDAC6 as a novel player in the angiogenic process and offer novel insights into the molecular mechanism governing endothelial cell migration and angiogenesis. |
|
