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篇目详细内容 |
【篇名】 |
Genetic diversity studies of coarse and fine rice using RAPD markers |
【刊名】 |
Frontiers of Agriculture in China |
【刊名缩写】 |
Front. Agric. China |
【ISSN】 |
1673-7334 |
【EISSN】 |
1673-744X |
【DOI】 |
10.1007/s11703-011-1106-2 |
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
【出版年】 |
2011 |
【卷期】 |
5
卷2期 |
【页码】 |
129-134
页,共
6
页 |
【作者】 |
Saeed ARSHAD;
Ahsan IQBAL;
Sehar NAWAZ;
Nisar AHMED;
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【关键词】 |
RAPD; diversity studies; genetic markers; fine rice; coarse rice |
【摘要】 |
The availability of a genetically diverse gene pool is vitally important in varietal development. Molecular markers are being extensively utilized to explore the genetic diversity among native and exotic germplasm. This study was designed to reveal the genetic diversity and patterns of relationships among the 20 accessions/genotypes representative of basmati and non-basmati rice from the existing rice gene pool using RAPD markers. Employing RAPD, 17 decamer oligonucleotide primers directed the amplification of 116 fragments, out of which 101 were polymorphic (87.06%) while 15 fragments were monomorphic (12.93%). Similarity coefficients had ranged from 0.47 to 0.90. The average genetic similarity was calculated 0.68 (68%). In this study, the coarse rice genotypes showed more polymorphism (85.84%) than the fine rice genotypes (61.76%). Genotypes were clustered into 8 distinct groups: A, B, C, D, E, F, G, and H but two genotypes, i.e., Shadab and Kangni-27 showed divergence from all the genotypes of the groups. Therefore, these diverse genotypes may be included in future breeding programmes. |
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