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篇目详细内容 |
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【篇名】 |
mRNA level of PKA-c gene in Setosphaeria turcica with different nutrition sources under metal ion or osmotic stress |
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【刊名】 |
Frontiers of Agriculture in China |
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【刊名缩写】 |
Front. Agric. China |
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【ISSN】 |
1673-7334 |
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【EISSN】 |
1673-744X |
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【DOI】 |
10.1007/s11703-011-1109-z |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2011 |
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【卷期】 |
5
卷3期 |
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【页码】 |
361-365
页,共
5
页 |
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【作者】 |
Qian WANG;
Wei ZHAO;
Zhimin HAO;
Jingao DONG;
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【关键词】 |
Setosphaeria turcica; PKA; gene expression; semiquantitative RT-PCR |
【摘要】 |
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Intracellular signal transduction pathways including MAPK, Ca2+, and cAMP signal transduction pathways play important roles in regulating growth, development, and pathogenesis of phytopathogenic fungi. Protein kinase A (PKA) is a key enzyme in cAMP signaling pathway. The transcription level of the gene encoding catalytic subunit of PKA in Setosphaeria turcica under different culture conditions was analyzed by the semiquantitative RT-PCR method. The expression level of PKA-c gene was the lowest on the medium containing sucrose and starch as the carbon source, and it was distinctly inhibited by Cu2+, but it was independent of nitrogen source. After the addition of different concentrations of sorbitol, it showed the positive correlation between the inhibition affection and concentrations. However, the highest expression was observed in response to NaCl (0.9？mol/L). This research enriched the biological information resource of filamentous fungi and laid a foundation for the functional analysis about signal transduction pathway in phytopathogenic fungi. |
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