|
|
|
篇目详细内容 |
|
【篇名】 |
Current molecular biologic techniques for characterizing environmental microbial community |
|
【刊名】 |
Frontiers of Environmental Science and Engineering |
|
【刊名缩写】 |
Front.Environ.Sci.Eng. |
|
【ISSN】 |
2095-2201 |
|
【EISSN】 |
2095-221X |
|
【DOI】 |
10.1007/s11783-011-0306-6 |
|
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
|
【出版年】 |
2012 |
|
【卷期】 |
6
卷1期 |
|
【页码】 |
82-97
页,共
16
页 |
|
【作者】 |
Dawen GAO;
Yu TAO;
|
|
【关键词】 |
molecular biological technique; microbial community; denaturing gradient gel electrophoresis (DGGE); terminal restriction fragment length polymorphism (T-RFLP); environmental applications |
【摘要】 |
|
Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant (WWTP). For the past 30 years, molecular biologic techniques explored for environmental microbial community (EMC) have spanned a broad range of approaches to facilitate the researches with the assistance of computer science: faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools. |
|
