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篇目详细内容 |
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【篇名】 |
Applications of HPLC-MS in compound Ilex pubescens extract study |
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【刊名】 |
Frontiers of Chemistry in China |
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【刊名缩写】 |
Front. Chem. China |
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【ISSN】 |
1673-3495 |
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【EISSN】 |
1673-3614 |
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【DOI】 |
10.1007/s11458-006-0005-1 |
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【出版社】 |
Higher Education Press and Springer-Verlag |
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【出版年】 |
2006 |
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【卷期】 |
1
卷2期 |
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【页码】 |
138-144
页,共
7
页 |
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【作者】 |
Yang Yunyun;
Hongwei Chen;
Zhong Ming;
Ma Zhiling;
Teng Jiuwei;
Mu Dehai;
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【关键词】 |
high performance liquid chromatography - mass spectrometry (HPLC-MS); compound Ilex pubescens; Chinese medicine herb; fingerprint; active components; electro spray ionization (ESI); atmospheric pressure chemical ionization (APCI) |
【摘要】 |
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In this paper, high performance liquid chromatography (HPLC) along with mass spectrometry (MS) and HPLC along with a diode array detector (DAD) was used to study the compound Ilex pubescens extract. Two ionization techniques: electro spray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were used in this work. The liquid chromatograms obtained by DAD, total ion chromatograms (TIC) from positive- and negative-ion ESI-MS and the positive- and negative-ion APCI-MS were compared. The liquid chromatograms obtained by TIC from ESI-MS provided more information on chromatographic peaks than those obtained by DAD or TIC from APCI-MS. It is suggested that the fingerprints of the compound Ilex pubescens extract should be provided by the liquid chromatograms obtained by DAD together with TIC from the negative-ion ESI-MS. The molecular weights of the nine main components in an HPLC-DAD chromatogram were determined by the corresponding positive- and negative-ion ESI and the positive- and negative-ion APCI mass spectra information. In the liquid chromatogram obtained by TIC from the negative-ion ESI-MS, the molecular weights of 23 main components were determined based on the corresponding positive- and negative-ion ESI mass spectra information. |
