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篇目详细内容 |
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【篇名】 |
Improvement of plant cryosection |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-012-2127-9 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2012 |
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【卷期】 |
7
卷4期 |
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【页码】 |
374-377
页,共
4
页 |
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【作者】 |
Zhaopei ZHANG;
Liyuan NIU;
Xiling CHEN;
Xu XU;
Zhengang RU;
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【关键词】 |
cryosection; wheat; histochemistry |
【摘要】 |
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Cryosection in plants is usually challenging because of the larger amounts of water contained in plant cell than animal cell. The formation of ice crystals within the plant cells easily destroys subcellular detail during freeze processing. And cell walls make freezing plant tissues hardly sliced and preserve the cell structure in subtle ways. In this study, the technique was improved on infusion, embedding and flatting sections with the winter wheat Variety Aikang58 as raw material. |
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