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篇目详细内容 |
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【篇名】 |
Functional protein microarray: an ideal platform for investigating protein binding property |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-012-1236-9 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2012 |
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【卷期】 |
7
卷4期 |
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【页码】 |
336-349
页,共
14
页 |
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【作者】 |
Shu-Min ZHOU;
Li CHENG;
Shu-Juan GUO;
Heng ZHU;
Sheng-Ce TAO;
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【关键词】 |
lectin microarray; protein microarray; protein-cell interaction; protein-DNA interaction (PDI); protein-protein interaction (PPI) |
【摘要】 |
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Functional protein microarray is an important tool for high-throughput and large-scale systems biology studies. Besides the progresses that have been made for protein microarray fabrication, significant advancements have also been achieved for applying protein microarrays on determining a variety of protein biochemical activities. Among these applications, detection of protein binding properties, such as protein-protein interactions (PPIs), protein-DNA interactions (PDIs), protein-RNA interactions, and antigen-antibody interactions, are straightforward and have substantial impacts on many research fields. In this review, we will focus on the recent progresses in protein-protein, protein-DNA, protein-RNA, protein-small molecule, protein-lipid, protein-glycan, and antigen-antibody interactions. We will also discuss the challenges and future directions of protein microarray technologies. We strongly believe that protein microarrays will soon become an indispensible tool for both basic research and clinical applications. |
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