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篇目详细内容 |
【篇名】 |
Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor |
【刊名】 |
Frontiers of Environmental Science and Engineering |
【刊名缩写】 |
Front.Environ.Sci.Eng. |
【ISSN】 |
2095-2201 |
【EISSN】 |
2095-221X |
【DOI】 |
10.1007/s11783-012-0393-z |
【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
【出版年】 |
2012 |
【卷期】 |
6
卷6期 |
【页码】 |
831-838
页,共
8
页 |
【作者】 |
Yuanting LI;
Dawei LI;
Wei SONG;
Meng LI;
Jie ZOU;
Yitao LONG;
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【关键词】 |
on-site determination; tyrosinase biosensor; phenolic contaminants; single-walled carbon nanotubes; gold nanoparticles; screen-printed electrodes |
【摘要】 |
A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10?8 to 2.0 × 10?5?mol·L?1 with a detection limit of 4.5 × 10?8?mol·L?1 (S/N = 3). This method has a rapid response time within 10?s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples. |
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