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篇目详细内容 |
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【篇名】 |
Current technologies to identify protein kinase substrates in high throughput |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-013-1257-z |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2013 |
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【卷期】 |
8
卷2期 |
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【页码】 |
216-227
页,共
12
页 |
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【作者】 |
Liang XUE;
W. Andy TAO;
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【关键词】 |
phosphorylation; kinase substrate; in vitro kinase assay; high throughput screening; mass spectrometry; phosphoproteomics |
【摘要】 |
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Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed. |
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