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篇目详细内容 |
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【篇名】 |
Comparative study of the cytotoxicity of the nanosized and microsized tellurium powders on HeLa cells |
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【刊名】 |
Frontiers in Biology |
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【刊名缩写】 |
Front. Biol.
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【ISSN】 |
1674-7984 |
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【EISSN】 |
1674-7992 |
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【DOI】 |
10.1007/s11515-013-1266-y |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2013 |
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【卷期】 |
8
卷4期 |
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【页码】 |
444-450
页,共
7
页 |
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【作者】 |
Huanan WEN;
Jiaxin ZHONG;
Bei SHEN;
Tao GAN;
Chao FU;
Zhihong ZHU;
Rui LI;
Xu YANG;
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【关键词】 |
nanosized and microsized tellurium powder; HeLa cells; oxidative damage; reactive oxygen species (ROS); glutathione (GSH); DNA and protein crosslink (DPC); 8-hydroxy-2’-deoxyguanosine (8-OHdG) |
【摘要】 |
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To compare the cytotoxicity on HeLa cells induced by nanosized and microsized tellurium powders, HeLa cells were exposed to different concentrations of tellurium powders (0, 50, 100, 150 and 200？μg/mL) for 12？h. In this study, detection of a series of biomarkers, including reactive oxygen species (ROS), glutathione (GSH), 8-hydroxy-2’-deoxyguanosine (8-OHdG), in addition to DNA and protein crosslink (DPC) and MTT assay, were conducted to evaluate the cytotoxicity. It is indicated that compared with the control group, there was no significant difference in the induced cytotoxicity at concentrations lower than 50？μg/mL for both nanosized and microsized tellurium powders. While there appears a significant difference in the induced cytotoxicity for nanosized tellurium powders when the concentration is higher than 100？μg/mL as well as for microsized tellurium powders when the concentration is higher than 200？μg/mL. Moreover, it is found that the cytotoxicity induced on HeLa cells exhibits a certain dose-effect relationship with the concentration of tellurium powders. A conclusion has been reached that the toxicity on HeLa cells can be induced by both nanosized and microsized tellurium powders, and the toxicity of the nanosized tellurium powders is significantly greater than the microsized one. |
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