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篇目详细内容 |
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【篇名】 |
Application of statistical design for the production of inulinase by streptomyces sp. using pressmud |
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【刊名】 |
Frontiers of Chemical Science and Engineering |
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【刊名缩写】 |
Front. Chem. Sci. Eng |
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【ISSN】 |
2095-0179 |
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【EISSN】 |
2095-0187 |
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【DOI】 |
10.1007/s11705-011-1112-1 |
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【出版社】 |
Higher Education Press and Springer-Verlag Berlin
Heidelberg |
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【出版年】 |
2011 |
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【卷期】 |
5
卷4期 |
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【页码】 |
463-470
页,共
8
页 |
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【作者】 |
M. DILIPKUMAR;
M. RAJASIMMAN;
N. RAJAMOHAN;
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【关键词】 |
inulinase; pressmud; Response Surface Methodology (RSM); streptomyces sp |
【摘要】 |
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A Plackett-Burman design was employed for screening 18 nutrient components for the production of inulinase using streptomyces sp. and pressmud as the substrate via solid-state fermentation (SSF). From the experiments, three nutrients viz. yeast extract, FeSO4·7H2O, and NH4NO3 were found to be the most significant components. Hence these three components were selected and optimized using Response Surface Methodology (RSM). The optimum conditions are: yeast extract 0.00274？g/gds, FeSO4·7H2O 0.00011？g/gds and NH4NO3 0.00772？g/gds. The effect of the substrate concentration and initial moisture content were also studied. A substrate concentration of 12？g and an initial moisture content of 65% are optimum for the maximum production of inulinase (89？U/gds). |
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